HLTF antibody immunoprecipitates HLTF protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HLTF protein by 2 μg of HLTF antibody (GTX114776) 7.5% SDS-PAGE The immunoprecipitated HLTF protein was detected by HLTF antibody (GTX114776) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114776 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF(GTX114776) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HLTF(GTX114776) antibody at 1:500 dilution.