GeneTex
United States (US)

HLTF antibody [N2C1], Internal

GTX114776_40233_IP_18121410_660.jpg
GTX114776_40233_IHC_18121410_348.jpg
GTX114776_40233_IFA_18121410_273.jpg
GTX114776_WB_18121410_690.jpg

Cat No. GTX114776

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLTF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Helicase Like Transcription Factor,Hip116,Hip116A,Hltf1,Rnf80,Smarca3,Snf2L3,Zbu1,Hltf

Cellular Localization

Nucleus

Background

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114776_40233_IP_18121410_660.jpg

GTX114776 IP Image

HLTF?antibody immunoprecipitates HLTF?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HLTF?protein by 2 μg of HLTF?antibody (GTX114776) 7.5% SDS-PAGE The immunoprecipitated HLTF?protein was detected by HLTF?antibody (GTX114776) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114776_40233_IHC_18121410_348.jpg

GTX114776 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF(GTX114776) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114776_40233_IFA_18121410_273.jpg

GTX114776 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HLTF(GTX114776) antibody at 1:500 dilution.

GTX114776_WB_18121410_690.jpg

GTX114776 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114776 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf