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HMBOX1 antibody [N3C3]

Anti-HMBOX1 antibody [N3C3] used in Western Blot (WB). GTX104189
Anti-HMBOX1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104189
Anti-HMBOX1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104189

Cat. No. GTX104189

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HMBOX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMBOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036940

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

homeobox containing 1 , HNF1LA , HOT1 , PBHNF

Cellular Localization

Nucleus

Background

Transcription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor.

Database

Research Area

DATA IMAGES

Anti-HMBOX1 antibody [N3C3] used in Western Blot (WB). GTX104189

GTX104189 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX104189 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMBOX1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104189

GTX104189 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Homeobox-containing protein 1(GTX104189) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMBOX1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104189

GTX104189 ICC/IF Image

HMBOX1 antibody [N3C3] detects HMBOX1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] (GTX104189) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Feng X et al. J Cell Sci 2013; 126 (Pt 17) : 760680 The telomere-associated homeobox-containing protein TAH1/HMBOX1 participates in telomere maintenance in ALT cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169