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HMBOX1 antibody [N3C3]

Anti-HMBOX1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104189
Anti-HMBOX1 antibody [N3C3] used in Western Blot (WB). GTX104189
Anti-HMBOX1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104189

Cat No. GTX104189

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMBOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036940

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

homeobox containing 1 , HNF1LA , HOT1 , PBHNF

Cellular Localization

Nucleus

Background

Transcription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor.

Database

Research Area

DATA IMAGES

Anti-HMBOX1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104189

GTX104189 ICC/IF Image

HMBOX1 antibody [N3C3] detects HMBOX1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] (GTX104189) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-HMBOX1 antibody [N3C3] used in Western Blot (WB). GTX104189

GTX104189 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX104189 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMBOX1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104189

GTX104189 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Homeobox-containing protein 1(GTX104189) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf