GeneTex
United States (US)

HMBOX1 antibody [N3C3]

GTX104189_40121_20150410_IFA_19011506_707.jpg
GTX104189_WB_19011506_746.jpg
GTX104189_IHC_19011506_366.jpg

Cat No. GTX104189

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMBOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Homeobox Containing 1,Hnf1La,Hot1,Pbhnf,Hmbox1

Cellular Localization

Nucleus

Background

Transcription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor.

Database

DATA IMAGES
GTX104189_40121_20150410_IFA_19011506_707.jpg

GTX104189 ICC/IF Image

HMBOX1 antibody [N3C3] detects HMBOX1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] (GTX104189) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX104189_WB_19011506_746.jpg

GTX104189 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX104189 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104189_IHC_19011506_366.jpg

GTX104189 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Homeobox-containing protein 1(GTX104189) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169