IHC-P analysis of human skin tissue using GTX85600 HMG14 (phospho Ser20/Ser24) antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 20 μg/ml

WB analysis of various sample lysates using GTX85600 HMG14 (phospho Ser20/Ser24) antibody. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues.
Lane 1 : HMGN1, non-phosphorylated
Lane 2 : HMGN1, phosphorylated
Lane 3 : HMGN1 delta20E, delta24E, non-phosphorylated
Lane 4 : HMGN1, delta20E, delta24E, phosphorylated
Lana 5 : HMGN2, non-phosphorylated
Lane 6 : HMGN2, phosphorylated
Dilution : 1:1000