GeneTex
United States (US)

HMGA1 (aa9-21) antibody, Internal (near N-term)

GTX88373_996_IHC-P_18121411_273.jpg
GTX88373_2400_WB_18121411_159.jpg
GTX88373_997_IHC-P_18121411_875.jpg

Cat No. GTX88373

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88373-PEP).

Specificity/Sensitivity

This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SQPLASKQEKDGT, from the internal region (near N Terminus) of the protein sequence according to NP_665906.1; NP_002122.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

High Mobility Group At-Hook 1,Hmg-R,Hmga1A,Hmgiy,Hmga1

Background

This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88373_996_IHC-P_18121411_273.jpg

GTX88373 IHC-P Image

IHC-P analysis of human placenta using GTX88373 HMGA1 (aa9-21) antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88373_2400_WB_18121411_159.jpg

GTX88373 WB Image

WB analysis of human pancreas lysate with (B) and without (A) blocking with the immunising peptide using GTX88373 HMGA1 (aa9-21) antibody, Internal (near N-term).
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

GTX88373_997_IHC-P_18121411_875.jpg

GTX88373 IHC-P Image

IHC-P analysis of human skin using GTX88373 HMGA1 (aa9-21) antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml