APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88373-PEP).
Product Note
This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SQPLASKQEKDGT, from the internal region (near N Terminus) of the protein sequence according to NP_665906.1; NP_002122.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
high mobility group AT-hook 1 , HMG-R , HMGA1A , HMGIY
Cellular Localization
Nucleus
Background
This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
|
GTX88373 IHC-P Image
IHC-P analysis of human skin using GTX88373 HMGA1 (aa9-21) antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
|
|
|
GTX88373 IHC-P Image
IHC-P analysis of human placenta using GTX88373 HMGA1 (aa9-21) antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
|
|
|
GTX88373 WB Image
WB analysis of human pancreas lysate with (B) and without (A) blocking with the immunising peptide using GTX88373 HMGA1 (aa9-21) antibody, Internal (near N-term).
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for HMGA1 (aa9-21) antibody, Internal (near N-term) (GTX88373). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HMGA1 (aa9-21) antibody, Internal (near N-term) (GTX88373). Be the first to share your experience with this product.
