Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:3000.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:1000.

HMGA1 antibody detects HMGA1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA1 protein stained by HMGA1 antibody (GTX132110) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.