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HMGA1 antibody

KO/KD
Anti-HMGA1 antibody used in Western Blot (WB). GTX132110
Anti-HMGA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132110
Anti-HMGA1 antibody used in Western Blot (WB). GTX132110

Cat. No. GTX132110

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HMGA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HMGA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886569

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group AT-hook 1 , HMG-R , HMGA1A , HMGIY

Cellular Localization

Nucleus

Background

This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGA1 antibody used in Western Blot (WB). GTX132110

GTX132110 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:3000.

Anti-HMGA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132110

GTX132110 ICC/IF Image

HMGA1 antibody detects HMGA1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA1 protein stained by HMGA1 antibody (GTX132110) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGA1 antibody used in Western Blot (WB). GTX132110

GTX132110 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:1000.

REFERENCE

Application Reference

Su L et al. Sci Rep 2020; 10 (1) : 18850 Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169