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HMGA2 antibody - ChIP grade

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Immunoprecipitation (IP). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

Cat No. GTX100519

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , HCT116

Predict Reactivity

Zebrafish, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.083 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Due to intellectual property restrictions, this product is not available for purchase in AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE.
TARGET

Synonyms

high mobility group AT-hook 2,BABL,HMGI-C,HMGIC,LIPO,STQTL9

Cellular Localization

Nucleus

Background

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100519

GTX100519 ICC/IF Image

HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA2 protein stained by HMGA2 antibody (GTX100519) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HMGA2 antibody (GTX100519) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
HMGA2 antibody (GTX100519) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody - ChIP grade used in Immunoprecipitation (IP). GTX100519

GTX100519 IP Image

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody (GTX100519).
Western blot analysis was performed using HMGA2 antibody (GTX100519).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human HuCCT1 xenograft .
HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169