GeneTex
United States (US)

HMGA2 antibody - ChIP grade

KO/KD
GTX100519_39869_IHC_19011506_591.jpg
GTX100519_42165_20151106_IFA_19011506_621.jpg
GTX100519_42123_20160526_WB_shRNA_watermark_19011506_880.jpg
GTX100519_42123_20150702_WB_19011506_305.jpg
GTX100519_42123_20151013_IHC-P_R_19011506_903.jpg
GTX100519_42123_20151013_IHC-P_R_2_19011506_308.jpg
GTX100519_39869_IP_19011506_162.jpg
GTX100519_41731_20151227_IHC-P_19011506_615.jpg
GTX100519_39869_IHC_M_19011506_452.jpg
GTX100519_42123_20150618_WB_19011506_103.jpg

Cat No. GTX100519

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , HCT116

Predict Reactivity

Zebrafish, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.083mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Due to intellectual property restrictions, this product is not available for purchase in AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE.
TARGET

Synonyms

High Mobility Group At-Hook 2,Babl,Hmgi-C,Hmgic,Lipo,Stqtl9,Hmga2

Cellular Localization

Nucleus

Background

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100519_39869_IHC_19011506_591.jpg

GTX100519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100519_42165_20151106_IFA_19011506_621.jpg

GTX100519 ICC/IF Image

HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA2 protein stained by HMGA2 antibody (GTX100519) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX100519_42123_20160526_WB_shRNA_watermark_19011506_880.jpg

GTX100519 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100519_42123_20150702_WB_19011506_305.jpg

GTX100519 WB Image

HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100519_42123_20151013_IHC-P_R_19011506_903.jpg

GTX100519 IHC-P Image

HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HMGA2 antibody (GTX100519) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100519_42123_20151013_IHC-P_R_2_19011506_308.jpg

GTX100519 IHC-P Image

HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
HMGA2 antibody (GTX100519) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100519_39869_IP_19011506_162.jpg

GTX100519 IP Image

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody (GTX100519).
Western blot analysis was performed using HMGA2 antibody (GTX100519).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100519_41731_20151227_IHC-P_19011506_615.jpg

GTX100519 IHC-P Image

HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human HuCCT1 xenograft .
HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100519_39869_IHC_M_19011506_452.jpg

GTX100519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100519_42123_20150618_WB_19011506_103.jpg

GTX100519 WB Image

HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169