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HMGA2 antibody - ChIP grade

KO/KD Orthogonal
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Immunoprecipitation (IP). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100519
Anti-HMGA2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX100519
Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

Cat. No. GTX100519

Reference 23

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , HCT116

Predict Reactivity

Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950498

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Due to intellectual property restrictions, this product is not available for purchase in AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE.

TARGET

Synonyms

high mobility group AT-hook 2 , BABL , HMGI-C , HMGIC , LIPO , STQTL9

Cellular Localization

Nucleus

Background

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HMGA2 antibody (GTX100519) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
HMGA2 antibody (GTX100519) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human HuCCT1 xenograft .
HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100519

GTX100519 ICC/IF Image

HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA2 stained by HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGA2 antibody - ChIP grade used in Immunoprecipitation (IP). GTX100519

GTX100519 IP Image

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody (GTX100519).
Western blot analysis was performed using HMGA2 antibody (GTX100519) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody - ChIP grade detects HMGA2 - ChIP grade protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HMGA2 - ChIP grade stained by HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX100519

GTX100519 IHC-P Image

HMGA2 antibody - ChIP grade detects HMGA2 - ChIP grade protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
HMGA2 - ChIP grade stained by HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 27821801

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 27821801

Anti-HMGA2 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100519

GTX100519 ICC/IF Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 27821801

Anti-HMGA2 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX100519

GTX100519 ChIP assay Image

The data was published in the journal Sci Rep in 2017.PMID: 28533522

Anti-HMGA2 antibody - ChIP grade used in Western Blot (WB). GTX100519

GTX100519 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27821801

REFERENCE

Application Reference

Owosho AA et al. Int J Clin Exp Pathol 2022; 15 (2) : 63-71 HMGA2 Immunoexpression is frequent in salivary gland pleomorphic adenoma: immunohistochemical and molecular analyses of PLAG1 and HMGA2 in 25 cases.
Application : IHC-P
Reactivity : Human
Jie-Ning Li et al. Front Cell Dev Biol 2021; 9 : 671244 E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
Li D et al. Oncol Lett 2021; 22 (6) : 849 Regulatory effect between HMGA2 and the Wnt/β-catenin signaling pathway in the carcinogenesis of sporadic colorectal tubular adenoma.
Application : WB, IHC-P
Reactivity : Human
Liu HH et al. Biochem Biophys Res Commun 2021; 567 : 183-189 Multiple myeloma driving factor WHSC1 is a transcription target of oncogene HMGA2 that facilitates colon cancer proliferation and metastasis.
Application : WB
Tang C et al. Cell Death Dis 2021; 12 (5) : 422 HMGA1B/2 transcriptionally activated-POU1F1 facilitates gastric carcinoma metastasis via CXCL12/CXCR4 axis-mediated macrophage polarization.
Application : ChIP assay
Reactivity : Mouse
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REVIEW

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Application Reference
Owosho AA et al. Int J Clin Exp Pathol 2022; 15 (2):63-71 HMGA2 Immunoexpression is frequent in salivary gland pleomorphic adenoma: immunohistochemical and molecular analyses of PLAG1 and HMGA2 in 25 cases.
Application : IHC-P
Reactivity : Human
Jie-Ning Li et al. Front Cell Dev Biol 2021; 9:671244 E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
Li D et al. Oncol Lett 2021; 22 (6):849 Regulatory effect between HMGA2 and the Wnt/β-catenin signaling pathway in the carcinogenesis of sporadic colorectal tubular adenoma.
Application : WB, IHC-P
Reactivity : Human
Liu HH et al. Biochem Biophys Res Commun 2021; 567:183-189 Multiple myeloma driving factor WHSC1 is a transcription target of oncogene HMGA2 that facilitates colon cancer proliferation and metastasis.
Application : WB
Tang C et al. Cell Death Dis 2021; 12 (5):422 HMGA1B/2 transcriptionally activated-POU1F1 facilitates gastric carcinoma metastasis via CXCL12/CXCR4 axis-mediated macrophage polarization.
Application : ChIP assay
Reactivity : Mouse
Su L et al. Sci Rep 2020; 10 (1):18850 Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays.
Application : WB, ChIP assay
Reactivity : Human
Liu B et al. FASEB J 2020; An HMGA2-p62-ERα axis regulates uterine leiomyomas proliferation.
Application : WB
Lopez-Nunez O et al. Hum Pathol 2020; 104:30-41 New molecular insights into the pathogenesis of lipoblastomas: clinicopathologic, immunohistochemical, and molecular analysis in pediatric cases.
Application : IHC-P
Reactivity : Human
Wu Y et al. Nucleic Acids Res 2020; 48 (9):4858-4876 The regulation of acetylation and stability of HMGA2 via the HBXIP-activated Akt-PCAF pathway in promotion of esophageal squamous cell carcinoma growth.
Application : ICC/IF
Reactivity : Human
Chen CH et al. Biochem Biophys Res Commun 2020; 524 (4):1003-1009 Dicoumarol suppresses HMGA2-mediated oncogenic capacities and inhibits cell proliferation by inducing apoptosis in colon cancer.
Application : WB
Reactivity : Human
Li Y et al. Thorac Cancer 2020; 11 (3):686-692
Application : WB
Reactivity : Human
Leung SW et al. Cancers (Basel) 2019; 11 (10) An Integrated Bioinformatics Analysis Repurposes an Antihelminthic Drug Niclosamide for Treating HMGA2-Overexpressing Human Colorectal Cancer.
Application : WB
Reactivity : Human
Venkatachalam G et al. Nucleic Acids Res 2017; 45 (18):10564-10582 Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress.
Nana AW et al. Endocr Relat Cancer 2017; (Epub) Tetrac downregulates β-catenin and HMGA2 to promote the effect of resveratrol in colon cancer.
Application : ICC/IF, IHC-P
Reactivity : Human
Liu Y et al. Mol Med Rep 2017; 16 (4):5107-5112 Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells.
Application : WB
Reactivity : Human
Venkatachalam G et al. Nucleic Acids Research 2017; (Epub) Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress.
Application : WB
Reactivity : Rat
de Vasconcellos JF et al. J Transl Med 2017; 15 (1):169 Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells.
Application : WB
Reactivity : Human
Wang Y et al. J Biol Chem 2017; (Epub) The long noncoding RNA HULC promotes liver cancer by increasing the expression of the HMGA2 oncogene via sequestration of the microRNA-186.
Application : WB, IHC-P
Reactivity : Human
Sun J et al. Sci Rep 2017; 7 (1):2229 HMGA2 promotes vasculogenic mimicry and tumor aggressiveness by upregulating Twist1 in gastric carcinoma.
Application : ChIP assay
Reactivity : Human
Wang Y et al. Sci Rep 2017; 7 (1):2070 The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.
Jinlong Yin et al. Oncotarget. 2016; 7 (48) TRIM71 suppresses tumorigenesis via modulation of Lin28B-let-7-HMGA2 signaling
Application : WB
Reactivity : Human
Wang Y et al. Biochem Biophys Res Commun 2016; 480 (3):455-460 MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3'UTR.
Application : WB
Reactivity : Human
Lee SH et al. Biochim Biophys Acta 2014; 1839 (5):374-386 The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169