GeneTex
United States (US)

HMGB1 antibody [GT383]

KO/KD Overexpression
GTX628834_41225_20170705_IFA_R_18121411_542.jpg
GTX628834_41225_IHC_M_18121411_167.jpg
GTX628834_41225_20170829_IHC-Fr_M_18121411_918.jpg
GTX628834_41225_WB_18121411_768.jpg
GTX628834_41225_20160602_WB_shRNA_watermark_18121411_138.jpg
GTX628834_41225_IHC_18121411_744.jpg
GTX628834_41225_WB_R_18121411_756.jpg
GTX628834_41225_20181005_WB_B_18121411_145.jpg
GTX628834_41225_WB_M_18121411_378.jpg
GTX628834_41225_20170728_WB_M_R_18121411_474.jpg

Cat No. GTX628834

Host Mouse
Clonality Monoclonal
Clone Name GT383
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686A04236 antibody, HMG1 antibody, HMG3 antibody, SBP-1 antibody, HMGB1 antibody, HMG-1 antibody, Amphoterin antibody, high-mobility group box 1 antibody, high mobility group protein B1 antibody, high mobility group protein 1 antibody, Sulfoglucuronyl carbohydrate binding protein antibody, high-mobility group (nonhistone chromosomal) protein 1 antibody

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES
GTX628834_41225_20170705_IFA_R_18121411_542.jpg

GTX628834 ICC/IF Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX628834_41225_IHC_M_18121411_167.jpg

GTX628834 IHC-P Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX628834_41225_20170829_IHC-Fr_M_18121411_918.jpg

GTX628834 IHC-Fr Image

HMGB1 antibody [GT383] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

GTX628834_41225_WB_18121411_768.jpg

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628834_41225_20160602_WB_shRNA_watermark_18121411_138.jpg

GTX628834 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628834_41225_IHC_18121411_744.jpg

GTX628834 IHC-P Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX628834_41225_WB_R_18121411_756.jpg

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat-2 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628834_41225_20181005_WB_B_18121411_145.jpg

GTX628834 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628834_41225_WB_M_18121411_378.jpg

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628834_41225_20170728_WB_M_R_18121411_474.jpg

GTX628834 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SDS
PBS.pdf