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HMGB1 antibody [GT383]

KO/KD Overexpression
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628834
Anti-HMGB1 antibody [GT383] used in IHC (Frozen sections) (IHC-Fr). GTX628834
Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

Cat. No. GTX628834

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT383

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain , HMGB1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888060

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628834

GTX628834 ICC/IF Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-HMGB1 antibody [GT383] used in IHC (Frozen sections) (IHC-Fr). GTX628834

GTX628834 IHC-Fr Image

HMGB1 antibody [GT383] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834

GTX628834 IHC-P Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834

GTX628834 IHC-P Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

HM Wang et al. Research Square 2023; Ferulic acid alleviates inflammatory manifestations in atopic dermatitis through modulation of the TRPV1/HMGB1 signaling pathway
Application : WB
Nakamura T et al. Oncol Rep 2020; 44 (6) : 2547-2558 High mobility group box 1 induces bone pain associated with bone invasion in a mouse model of advanced head and neck cancer.
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations