HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HMGB1 antibody [GT383] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.