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HMGB1 antibody [GT383]

KO/KD Overexpression
Anti-HMGB1 antibody [GT383] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628834
Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834
Anti-HMGB1 antibody [GT383] used in IHC (Frozen sections) (IHC-Fr). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834
Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

Cat No. GTX628834

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT383

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888060

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody [GT383] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628834

GTX628834 ICC/IF Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834

GTX628834 IHC-P Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody [GT383] used in IHC (Frozen sections) (IHC-Fr). GTX628834

GTX628834 IHC-Fr Image

HMGB1 antibody [GT383] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in IHC (Paraffin sections) (IHC-P). GTX628834

GTX628834 IHC-P Image

HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody [GT383] (GTX628834) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT383] used in Western Blot (WB). GTX628834

GTX628834 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Nakamura T et al. Oncol Rep 2020; 44 (6) : 2547-2558 High mobility group box 1 induces bone pain associated with bone invasion in a mouse model of advanced head and neck cancer.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations