APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , mouse brain , rat brain
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.73 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888060
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Cellular Localization
Nucleus
Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Database
Research Area
DATA IMAGES
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GTX628834 ICC/IF Image
HMGB1 antibody [GT383] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:500. Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX628834 IHC-P Image
HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628834 IHC-Fr Image
HMGB1 antibody [GT383] detects HMGB1 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HMGB1 stained by HMGB1 antibody [GT383] (GTX628834) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min
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GTX628834 WB Image
HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 12% SDS-PAGE HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628834 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628834 IHC-P Image
HMGB1 antibody [GT383] detects HMGB1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. HMGB1 antibody [GT383] (GTX628834) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628834 WB Image
HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 12% SDS-PAGE HMGB1 antibody [GT383] (GTX628834) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628834 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628834 WB Image
HMGB1 antibody [GT383] detects HMGB1 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg BCL-1 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract F. 30 μg C2C12 whole cell lysate/extract 12% SDS-PAGE HMGB1 antibody [GT383] (GTX628834) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628834 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT383] (GTX628834) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
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