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HMGB1 antibody [GT427]

Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445
Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445
Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445
Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445
Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445
Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445
Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445
Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445

Cat No. GTX629445

Host

Mouse

Clonality

Monoclonal

Clone Name

GT427

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , SK-N-SH , IMR32 , SK-N-AS , Mouse brain , Rat brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888111

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445

GTX629445 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:2000.

Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445

GTX629445 ICC/IF Image

HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445

GTX629445 ICC/IF Image

HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445

GTX629445 IHC-Fr Image

HMGB1 antibody [GT427] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445

GTX629445 ICC/IF Image

HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445

GTX629445 WB Image

HMGB1 antibody [GT427] detects 0 protein by Western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody [GT427] (GTX629445) dilution: 1:1000

Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445

GTX629445 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445

GTX629445 WB Image

HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:1000.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations