Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:2000.

HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

HMGB1 antibody [GT427] detects 0 protein by Western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody [GT427] (GTX629445) dilution: 1:1000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:1000.

HMGB1 antibody [GT427] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).