APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain , 293T , HMGB1-transfected 293T
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888111
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Cellular Localization
Nucleus
Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Database
Research Area
DATA IMAGES
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/templates/default/js/lightbox/images/loading.gif "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
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GTX629445 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:2000.
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GTX629445 ICC/IF Image
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX629445 ICC/IF Image
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX629445 IHC-Fr Image
HMGB1 antibody [GT427] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min
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GTX629445 ICC/IF Image
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.
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GTX629445 WB Image
HMGB1 antibody [GT427] detects 0 protein by Western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody [GT427] (GTX629445) dilution: 1:1000
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GTX629445 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629445 WB Image
HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:1000.
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REFERENCE
REVIEW
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![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20160602_WB_shRNA_watermark_18121411_775.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20170727_IFA_R_18121411_141.jpg "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20170705_IFA_R_18121411_696.jpg "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20170829_IHC-Fr_M_18121411_508.jpg "Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20160824_IFA_18121411_797.jpg "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_WB_18121411_493.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20181005_WB_B_18121411_543.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20151015_WB_Merged_18121411_314.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20160602_WB_shRNA_watermark_18121411_775.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20170727_IFA_R_18121411_141.jpg "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20170705_IFA_R_18121411_696.jpg "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20170829_IHC-Fr_M_18121411_508.jpg "Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20160824_IFA_18121411_797.jpg "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_WB_18121411_493.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20181005_WB_B_18121411_543.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20151015_WB_Merged_18121411_314.jpg "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")