GeneTex
United States (US)

HMGB1 antibody [Giby-1-4]

GTX33926_20160831_Structure_18121410_842.jpg
GTX33926_20160831_WB_18121410_510.jpg

Cat No. GTX33926

Host Human
Clonality Monoclonal
Clone Name Giby-1-4
Isotype IgG2
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Western blot: 1:1000

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

Recognizes human, mouse and rat HMGB1.
PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant HMGB1.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX33926_20160831_Structure_18121410_842.jpg

GTX33926 Image

Schematic antibody structure.

GTX33926_20160831_WB_18121410_510.jpg

GTX33926 WB Image

Western blot analysis of human and rat HMGB1 using anti-HMGB1, mAb (rec.) (GIBY-1-4) Different amounts of cell extracts from HEK293T cells (3μg, 5μg and 30μg) either transfected with a plasmid coding for rat HMGB1 (lanes 1, 2, 3) or non-transfected (lanes 4, 5, 6), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-HMGB1,mAb (rec.) (GIBY-1-4) (1μg /ml). Proteins were visualized by a chemiluminescence detection system.

Package List Price ($)
$ 339