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HMGB1 antibody [HAP46.5]

Cat. No. GTX12029

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HAP46.5

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat, Dog, Chicken
Package
100 μl ($519)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

MCF7

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

purified recombinant human HMG-1.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_367629

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome

Background

HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. Both HMG1 and HMG2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMG1/2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites, such as steroid hormone receptors, p53, and Oct. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication.

Database

Research Area

REFERENCE

Application Reference

Romano E et al. Oncotarget 2018; 9 (25) : 17631-17644 BNIP3 modulates the interface between B16-F10 melanoma cells and immune cells.
Prasad R et al. Nucleic Acids Res 2012; 40 (22) : 11571-82 Pol β associated complex and base excision repair factors in mouse fibroblasts.
Yang J et al. J Cell Sci 2010; 123 (Pt 6) : 861-70 GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.
Application : WB
Reactivity : Human
Sun L et al. Cardiovasc Res 2009; 82 (2) : 371-81 RAGE mediates oxidized LDL-induced pro-inflammatory effects and atherosclerosis in non-diabetic LDL receptor-deficient mice.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519