APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:2000 |
1:100-1:300 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone J2E1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HMGB3 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
high mobility group box 1 , HMG1 , HMG3 , HMGB1 , SBP1
Cellular Localization
Nucleus,Chromosome,Cytoplasm,Secreted,Cell membrane,Endosome,Endoplasmic reticulum-Golgi intermediate compartment
Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
|
GTX57549 WB Image
WB analysis of various samples using GTX57549 HMGB1 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : Jurkat whole cell lysate Lane 3 : K562 whole cell lysate Lane 4 : A549 whole cell lysate Lane 5 : MCF-7 whole cell lysate Loading : 40 μg Dilution : 1:1000
|
|
GTX57549 IHC-P Image
IHC-P analysis of human colon cancer using GTX57549 HMGB1 antibody. Antigen retrieval: 0.1M sodium citrate buffer Dilution: 1:200
|
REFERENCE
There are currently no references for HMGB1 antibody [J2E1] (GTX57549). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HMGB1 antibody [J2E1] (GTX57549). Be the first to share your experience with this product.