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HMGB1 antibody

KO/KD Orthogonal Overexpression
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

Cat. No. GTX101277

Reference 21
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish, Pig
Package
100 μl ($399),
25 μl ($169)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950499

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277

GTX101277 IHC-Wm Image

HMGB1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by HMGB1 antibody (GTX101277) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HMGB1 antibody (GTX101277) at 1:300 dilution.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhengke Li et al. FASEB J 2021; 35 (5) : e21373 ATR prevents Ca2+ overload-induced necrotic cell death through phosphorylation-mediated inactivation of PARP1 without DNA damage signaling.
Application : WB
Reactivity : Human
Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.
Application : WB
Reactivity : Human
Sixto-L?pez Y et al. Int J Mol Sci 2020; 21 (16) N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells.
Kanno Y et al. Arthritis Res Ther 2020; 22 (1) : 76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.
Application : WB
Reactivity : Mouse
Huang L et al. Aging (Albany NY) 2019; 11 (17) : 6674-6690 RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways.
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Submit a Reference

REVIEW

HMGB1 antibody Cat. No. GTX101277

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2016 in Nat Commun. PMID: 27329339
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 30279967
Submit a Review
Application Reference
Zhengke Li et al. FASEB J 2021; 35 (5):e21373 ATR prevents Ca2+ overload-induced necrotic cell death through phosphorylation-mediated inactivation of PARP1 without DNA damage signaling.
Application : WB
Reactivity : Human
Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.
Application : WB
Reactivity : Human
Sixto-L?pez Y et al. Int J Mol Sci 2020; 21 (16) N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells.
Kanno Y et al. Arthritis Res Ther 2020; 22 (1):76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.
Application : WB
Reactivity : Mouse
Huang L et al. Aging (Albany NY) 2019; 11 (17):6674-6690 RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways.
D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17):E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.
Contis-Montes de Oca A et al. Oncotarget 2018; 9 (70):33368-33381 N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a Histone Deacetylase Inhibitor, Induces the Release of Nuclear HMGB1 and Modifies ROS Levels in HeLa Cells.
Coleman LG Jr et al. Brain Behav Immun 2017; (Epub) HMGB1/IL-1β complexes regulate neuroimmune responses in alcoholism.
Application : WB
Reactivity : Human
Masola V et al. FASEB J 2017; (Epub) Heparanase regulates the * polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury.
Application : ICC/IF
Reactivity : Human
Liu PL et al. PLoS One 2017; 12 (7):e0180844 MicroRNA-200c inhibits epithelial-mesenchymal transition, invasion, and migration of lung cancer by targeting HMGB1.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Lin CY et al. Cell Death Discov 2016; 2:16065 Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.
Application : WB
Reactivity : Human
Dick MS et al. Nat Commun 2016; 7:11929 ASC filament formation serves as a signal amplification mechanism for inflammasomes.
Application : WB
Reactivity : Mouse
Bhatia A et al. Mol Ther Methods Clin Dev 2016; 3:16041 Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs.
Application : IHC-P
Reactivity : Pig
Sundararaman B et al. Mol Cell 2016; 61 (6):903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF
Reactivity : Human
Lin TJ et al. Mol Cancer 2015; 14:174 Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components.
Application : WB
Reactivity : Mouse
Juranek JK et al. Front Cell Neurosci 2015; 9:485 Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis.
Application : WB
Reactivity : Human
Ruhl S et al. Eur J Immunol 2015; 45 (10):2927-36 Caspase-11 activates a canonical NLRP3 inflammasome by promoting K(+) efflux.
Application : WB
Reactivity : Human
Xu YF et al. World J Gastroenterol 2015; 21 (11):3256-65 High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma.
Application : IHC
Reactivity : Human
Lee SM et al. Int J Cardiol 2015; 190:353-9 Amitriptyline pharmacologically preconditions rat hearts against cardiac ischemic-reperfusion injury.
Application : WB
Reactivity : Rat
Kayagaki N et al. Nature 2011; 479 (7371):117-21 Non-canonical inflammasome activation targets caspase-11.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169