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HMGB1 antibody

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277
Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

Cat. No. GTX101277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, IHC, IHC (Free Floating)

Reactivity

Human, Mouse, Rat, Zebrafish, Pig
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IHC Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 antibody (GTX101277) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX101277

GTX101277 IHC-Wm Image

HMGB1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Hmgb1 stained by HMGB1 antibody (GTX101277) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101277

GTX101277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HMGB1 antibody (GTX101277) at 1:300 dilution.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

HMGB1 antibody detects HMGB1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
12% SDS-PAGE
HMGB1 antibody (GTX101277) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101277

GTX101277 ICC/IF Image

The data was published in the journal Oncotarget in 2018. PMID: 30279967

Anti-HMGB1 antibody used in Western Blot (WB). GTX101277

GTX101277 WB Image

The data was published in the 2016 in Nat Commun. PMID: 27329339

REFERENCE

REVIEW

HMGB1 antibody Cat. No. GTX101277

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Free Floating)(IHC (Free Floating)) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : adult mouse cerebral cortex tissue section
Blocking : 1% BSA in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100 / No antigen retireval
Primary Antibody : 1:500, 16°C, 4Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169