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HMGB1 antibody

KO/KD Overexpression
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112959
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112959
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112959
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112959

Cat No. GTX112959

Reference 1
1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all HMGB1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , HepG2 , mouse brain , PC-12 , Rat2 , *rat brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

High Mobility Group Box 1,Hmg-1,Hmg1,Hmg3,Sbp-1,Hmgb1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959

GTX112959 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112959

GTX112959 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using HMG-1(GTX112959) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959

GTX112959 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112959

GTX112959 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
HMGB1 antibody (GTX112959) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959

GTX112959 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112959 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959

GTX112959 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959

GTX112959 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112959

GTX112959 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
HMGB1 antibody (GTX112959) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959

GTX112959 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX112959 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112959

GTX112959 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX112959) diluted at 1:1000.
Blue: Hoechst 33342 staining.
REFERENCE

Application Reference

Yang C et al. PLoS One 2011; 6 (8) : e22517 Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model.
Application : WB
Reactivity : Mouse
Submit a Reference
REVIEW

HMGB1 antibody Cat No. GTX112959

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-HMGB1 antibody used in Western Blot (WB). GTX112959
Date : Anonymous submitted on 16-Oct-2012
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain primary astrocyte tissue
Amount used : 30μg
Blocking : 5% milk , 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169