APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HepG2 , mouse brain , PC-12 , Rat2 , *rat brain
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.53 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950500
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Cellular Localization
Nucleus
Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Database
Research Area
DATA IMAGES
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GTX112959 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112959 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using HMG-1(GTX112959) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112959 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112959 IHC-P Image
HMGB1 antibody detects HMGB1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
HMGB1 antibody (GTX112959) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112959 WB Image
Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112959 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112959 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112959 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX112959) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112959 IHC-P Image
HMGB1 antibody detects HMGB1 protein at nucleus on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
HMGB1 antibody (GTX112959) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112959 WB Image
Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX112959 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112959 ICC/IF Image
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX112959) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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REFERENCE
REVIEW
HMGB1 antibody
Cat No. GTX112959
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 16-Oct-2012
Application Tested :
WB
Sample Species :
Rat
Sample :
Rat brain primary astrocyte tissue
Amount used :
30μg
Blocking :
5% milk , 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
