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HMGB1 antibody

KO/KD Overexpression
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in Immunoprecipitation (IP). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344
Anti-HMGB1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

Cat. No. GTX127344

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

mouse brain , rat brain , 293T , HMGB1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164700

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:5000.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344

GTX127344 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
HMGB1 antibody (GTX127344) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.

Anti-HMGB1 antibody used in Immunoprecipitation (IP). GTX127344

GTX127344 IP Image

Immunoprecipitation of HMGB1 protein from 293T whole cell extracts using 5 μg of HMGB1 antibody (GTX127344).
Western blot analysis was performed using HMGB1 antibody (GTX127344).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
HMGB1 antibody (GTX127344) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344

GTX127344 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Anti-HMGB1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127344

GTX127344 IHC-Fr Image

HMGB1 antibody detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using HMGB1(GTX127344) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344

GTX127344 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kubota R et al. Br J Pharmacol 2020; 177 (22) : 5195-5207 Inhibition of γ-glutamyltransferase ameliorates ischaemia-reoxygenation tissue damage in rats with hepatic steatosis.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

HMGB1 antibody Cat. No. GTX127344

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-HMGB1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127344
Date : Anonymous submitted on 24-Aug-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Inflammed myocardium tissue
Blocking : 10 % Horse serum in PBS, 25°C, 1Hr
Fixation : 4% PFA in PBS
Primary Antibody : 1:250, 25°C, 1Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169