APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse brain , rat brain
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.59 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
High Mobility Group Box 1,Hmg-1,Hmg1,Hmg3,Sbp-1,Hmgb1
Cellular Localization
Nucleus
Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Database
Research Area
DATA IMAGES
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GTX127344 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:5000.
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GTX127344 ICC/IF Image
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX127344 IHC-P Image
HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
HMGB1 antibody (GTX127344) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127344 WB Image
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.
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GTX127344 IP Image
Immunoprecipitation of HMGB1 protein from 293T whole cell extracts using 5 μg of HMGB1 antibody (GTX127344).
Western blot analysis was performed using HMGB1 antibody (GTX127344).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX127344 IHC-P Image
HMGB1 antibody detects HMGB1 protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
HMGB1 antibody (GTX127344) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127344 WB Image
HMGB1 antibody detects HMGB1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody (GTX127344) dilution: 1:1000
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GTX127344 ICC/IF Image
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
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GTX127344 IHC-Fr Image
HMGB1 antibody detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min
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GTX127344 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using HMGB1(GTX127344) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127344 WB Image
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.
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GTX127344 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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