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HMGB1 antibody

KO/KD Overexpression
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in Immunoprecipitation (IP). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344
Anti-HMGB1 antibody used in Western Blot (WB). GTX127344
Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

Cat. No. GTX127344

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, RIP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
RIP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

mouse brain , rat brain , 293T , HMGB1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus

Background

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:5000.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
HMGB1 antibody (GTX127344) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.

Anti-HMGB1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127344

GTX127344 IHC-Fr Image

HMGB1 antibody detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
HMGB1 antibody (GTX127344) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344

GTX127344 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344

GTX127344 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-HMGB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127344

GTX127344 ICC/IF Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using HMGB1(GTX127344) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGB1 antibody used in Immunoprecipitation (IP). GTX127344

GTX127344 IP Image

Immunoprecipitation of HMGB1 protein from 293T whole cell extracts using 5 μg of HMGB1 antibody (GTX127344).
Western blot analysis was performed using HMGB1 antibody (GTX127344).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGB1 antibody used in Western Blot (WB). GTX127344

GTX127344 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.

Anti-HMGB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127344

GTX127344 IHC-P Image

HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

L Li et al. Pharmacol Res 2023; Circulating extracellular vesicles from patients with traumatic brain injury induce cerebrovascular endothelial dysfunction
Application : RIP
Kubota R et al. Br J Pharmacol 2020; 177 (22) : 5195-5207 Inhibition of γ-glutamyltransferase ameliorates ischaemia-reoxygenation tissue damage in rats with hepatic steatosis.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

HMGB1 antibody Cat. No. GTX127344

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-HMGB1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX127344
Date : Anonymous submitted on 24-Aug-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Inflammed myocardium tissue
Blocking : 10 % Horse serum in PBS, 25°C, 1Hr
Fixation : 4% PFA in PBS
Primary Antibody : 1:250, 25°C, 1Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169