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HMGB2 antibody

KO/KD Orthogonal Overexpression
Anti-HMGB2 antibody used in Western Blot (WB). GTX129464
Anti-HMGB2 antibody used in Western Blot (WB). GTX129464
Anti-HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129464
Anti-HMGB2 antibody used in Western Blot (WB). GTX129464

Cat. No. GTX129464

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HMGB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human HMGB1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human HMGB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 2 , HMG2

Cellular Localization

Nucleus

Background

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGB2 antibody used in Western Blot (WB). GTX129464

GTX129464 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:2000.

Anti-HMGB2 antibody used in Western Blot (WB). GTX129464

GTX129464 WB Image

HMGB2 antibody detects HMGB2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
HMGB2 antibody (GTX129464) dilution: 1:1000

Anti-HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129464

GTX129464 IHC-P Image

HMGB2 antibody detects HMGB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HMGB2 antibody (GTX129464) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB2 antibody used in Western Blot (WB). GTX129464

GTX129464 WB Image

Overexpression

HMGB2 antibody detects HMGB2 protein by western blot analysis. Non-transfected (-), HMGB1-transfected (+) and HMGB2-transfected (+) whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:3000.

REFERENCE

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REVIEW

HMGB2 antibody Cat. No. GTX129464

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-HMGB2 antibody used in Western Blot (WB). GTX129464
Date : Anonymous submitted on 22-Jan-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa chormatin bound fractions with/without other siRNA target treatment
Amount used : 10μg
Blocking : BSA, 26°C, 0.1Hr
Primary Antibody : 1:1000, °C, Hr
Anti-HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129464
Date : Anonymous submitted on 22-Jan-2024
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : BSA, 26°C, 0.1Hr
Fixation : 4% PFA
Primary Antibody : 1:1000, 26°C, 0.5Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169