APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , human HMGB1-transfected 293T , human HMGB2-transfected 293T , HMGB2-transfected 293T
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human HMGB2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886004
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
high mobility group box 2 , HMG2
Cellular Localization
Nucleus
Background
This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129464 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:2000.
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GTX129464 IHC-P Image
HMGB2 antibody detects HMGB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HMGB2 antibody (GTX129464) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129464 WB Image
HMGB2 antibody detects HMGB2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
HMGB2 antibody (GTX129464) dilution: 1:1000
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GTX129464 WB Image
HMGB2 antibody detects HMGB2 protein by western blot analysis. Non-transfected (-), HMGB1-transfected (+) and HMGB2-transfected (+) whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:3000.
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REFERENCE
There are currently no references for HMGB2 antibody (GTX129464). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HMGB2 antibody (GTX129464). Be the first to share your experience with this product.
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