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HMGB2 antibody

KO/KD Overexpression
Anti-HMGB2 antibody used in Western Blot (WB). GTX129469
Anti-HMGB2 antibody used in Western Blot (WB). GTX129469
Anti-HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129469
Anti-HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129469
Anti-HMGB2 antibody used in Western Blot (WB). GTX129469

Cat. No. GTX129469

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HMGB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HMGB2-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HMGB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886008

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 2 , HMG2

Cellular Localization

Nucleus

Background

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGB2 antibody used in Western Blot (WB). GTX129469

GTX129469 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129469) diluted at 1:1000.

Anti-HMGB2 antibody used in Western Blot (WB). GTX129469

GTX129469 WB Image

HMGB2 antibody detects HMGB2 protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg A431
C. 30 μg HeLa
D. 30 μg HepG2
12 % SDS-PAGE
HMGB2 antibody (GTX129469) dilution: 1:1000

Anti-HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129469

GTX129469 ICC/IF Image

HMGB2 antibody detects HMGB2 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB2 protein stained by HMGB2 antibody (GTX129469) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129469

GTX129469 IHC-P Image

HMGB2 antibody detects HMGB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HMGB2 antibody (GTX129469) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB2 antibody used in Western Blot (WB). GTX129469

GTX129469 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129469) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169