GeneTex
United States (US)

HMGB3 antibody, Internal

GTX88447_2310_WB_18121411_926.jpg

Cat No. GTX88447

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88447-PEP).

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KFDGAKGPAKVARKK, from the internal region (near C Terminus) of the protein sequence according to NP_005333.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

High Mobility Group Box 3,Hmg-2A,Hmg-4,Hmg2A,Hmg4,Hmgb3

Background

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES
GTX88447_2310_WB_18121411_926.jpg

GTX88447 WB Image

WB analysis of human lung lysate using GTX88447 HMGB3 antibody, Internal.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329