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HMGCL antibody [N2C3]

Anti-HMGCL antibody [N2C3] used in Western Blot (WB). GTX114472
Anti-HMGCL antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114472

Cat No. GTX114472

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

3-Hydroxy-3-Methylglutaryl-Coa Lyase,Hl,Hmgcl

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HMGCL antibody [N2C3] used in Western Blot (WB). GTX114472

GTX114472 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
HL antibody
GTX114472 diluted at 1:1000

Anti-HMGCL antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114472

GTX114472 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using HMGCL(GTX114472) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169