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HMGCL antibody

Anti-HMGCL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109096
Anti-HMGCL antibody used in Western Blot (WB). GTX109096
Anti-HMGCL antibody used in Western Blot (WB). GTX109096
Anti-HMGCL antibody used in Western Blot (WB). GTX109096
Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX109096

Cat. No. GTX109096

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGCL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse liver , H1299 , rat liver

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HMGCL Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950501

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA lyase , HL

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGCL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109096

GTX109096 ICC/IF Image

HMGCL antibody detects HMGCL protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HMGCL protein stained by HMGCL antibody (GTX109096) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGCL antibody used in Western Blot (WB). GTX109096

GTX109096 WB Image

HMGCL antibody detects HMGCL protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (GTX109096) dilution: 1:10000

Anti-HMGCL antibody used in Western Blot (WB). GTX109096

GTX109096 WB Image

HMGCL antibody detects HMGCL protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (GTX109096) dilution: 1:10000

Anti-HMGCL antibody used in Western Blot (WB). GTX109096

GTX109096 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX109096 diluted at 1:3000

Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX109096

GTX109096 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL(GTX109096) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169