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HMGCL antibody

Anti-HMGCL antibody used in Western Blot (WB). GTX109096
Anti-HMGCL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109096
Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX109096
Anti-HMGCL antibody used in Western Blot (WB). GTX109096
Anti-HMGCL antibody used in Western Blot (WB). GTX109096

Cat. No. GTX109096

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGCL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse liver , H1299 , rat liver

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HMGCL Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950501

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA lyase , HL

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGCL antibody used in Western Blot (WB). GTX109096

GTX109096 WB Image

HMGCL antibody detects HMGCL protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (GTX109096) dilution: 1:10000

Anti-HMGCL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109096

GTX109096 ICC/IF Image

HMGCL antibody detects HMGCL protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HMGCL protein stained by HMGCL antibody (GTX109096) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX109096

GTX109096 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL(GTX109096) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGCL antibody used in Western Blot (WB). GTX109096

GTX109096 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX109096 diluted at 1:3000

Anti-HMGCL antibody used in Western Blot (WB). GTX109096

GTX109096 WB Image

HMGCL antibody detects HMGCL protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (GTX109096) dilution: 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169