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HMGCL antibody

Anti-HMGCL antibody used in Western Blot (WB). GTX33242
Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX33242
Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX33242
Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX33242

Cat. No. GTX33242

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HMGCL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human HMGCL (NP_000182.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA lyase , HL

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-HMGCL antibody used in Western Blot (WB). GTX33242

GTX33242 WB Image

WB analysis of various sample lysates using GTX33242 HMGCL antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX33242

GTX33242 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33242 HMGCL antibody.
Dilution : 1:100

Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX33242

GTX33242 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX33242 HMGCL antibody.
Dilution : 1:100

Anti-HMGCL antibody used in IHC (Paraffin sections) (IHC-P). GTX33242

GTX33242 IHC-P Image

IHC-P analysis of human kidney tissue using GTX33242 HMGCL antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399