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HMGCR antibody, Internal

Anti-HMGCR antibody, Internal used in Western Blot (WB). GTX88456

Cat. No. GTX88456

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all HMGCR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88456-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ADPSPQNSTADTSK , from the internal region of the protein sequence according to NP_000850.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722587

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA reductase , LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-HMGCR antibody, Internal used in Western Blot (WB). GTX88456

GTX88456 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28831159

REFERENCE

Application Reference

Nelson RK et al. Sci Rep 2017; 7 (1) : 9112 Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway.
Application : WB
Reactivity : Human
Lai YS et al. J Agric Food Chem 2014; Garlic essential oil protects against obesity-triggered nonalcoholic fatty liver disease through modulation of lipid metabolism and oxidative stress.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399