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HMGCR antibody

Anti-HMGCR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54088
Anti-HMGCR antibody used in Western Blot (WB). GTX54088
Anti-HMGCR antibody used in Western Blot (WB). GTX54088
Anti-HMGCR antibody used in IHC (Paraffin sections) (IHC-P). GTX54088
Anti-HMGCR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54088
Anti-HMGCR antibody used in IHC (Paraffin sections) (IHC-P). GTX54088
Anti-HMGCR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54088

Cat. No. GTX54088

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HMGCR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:4000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA reductase , LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-HMGCR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54088

GTX54088 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54088 HMGCR antibody.
Blue : DAPI
Dilution : 1:100

Anti-HMGCR antibody used in Western Blot (WB). GTX54088

GTX54088 WB Image

WB analysis of mouse spleen tissue lysate using GTX54088 HMGCR antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HMGCR antibody used in Western Blot (WB). GTX54088

GTX54088 WB Image

WB analysis of rat brain tissue lysate using GTX54088 HMGCR antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HMGCR antibody used in IHC (Paraffin sections) (IHC-P). GTX54088

GTX54088 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX54088 HMGCR antibody.
Dilution : 1:200

Anti-HMGCR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54088

GTX54088 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54088 HMGCR antibody.
Blue : DAPI
Dilution : 1:100

Anti-HMGCR antibody used in IHC (Paraffin sections) (IHC-P). GTX54088

GTX54088 IHC-P Image

IHC-P analysis of human uterine cancer tissue using GTX54088 HMGCR antibody.
Dilution : 1:200

Anti-HMGCR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54088

GTX54088 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54088 HMGCR antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Maria Giovanna Lupo et al. Int J Mol Sci 2021; 22 (8) PCSK9 Induces Rat Smooth Muscle Cell Proliferation and Counteracts the Pleiotropic Effects of Simvastatin.
Application : WB
Reactivity : Rat
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399