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HMGCS1 antibody

Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346
Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346
Anti-HMGCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112346
Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346
Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346

Cat No. GTX112346

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA synthase 1 , HMGCS

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346

GTX112346 WB Image

HMGCS1 antibody detects HMGCS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (GTX112346) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346

GTX112346 IHC-P Image

HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGCS1 antibody (GTX112346) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112346

GTX112346 ICC/IF Image

HMGCS1 antibody detects HMGCS1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGCS1 protein stained by HMGCS1 antibody (GTX112346) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346

GTX112346 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX112346 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346

GTX112346 IHC-P Image

HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGCS1 antibody (GTX112346) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf