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HMGCS2 antibody

Anti-HMGCS2 antibody used in Western Blot (WB). GTX128013
Anti-HMGCS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128013

Cat No. GTX128013

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

human HMGCS2-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA synthase 2

Cellular Localization

Mitochondrion

Background

Mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase (HMGCS2; EC 2.3.3.10) mediates the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for brain, heart, kidney, and other organs during times of carbohydrate deprivation such as fasting (Robinson and Williamson, 1980 [PubMed 6986618]). Also see cytoplasmic HMG-CoA synthase (HMGCS1; MIM 142940), which mediates an early step in cholesterol synthesis.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HMGCS2 antibody used in Western Blot (WB). GTX128013

GTX128013 WB Image

HMGCS2 antibody detects HMGCS2 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human HMGCS2-transfected 293T cells
7.5 % SDS-PAGE
HMGCS2 antibody (GTX128013) dilution: 1:5000

Anti-HMGCS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128013

GTX128013 IHC-P Image

HMGCS2 antibody detects HMGCS2 protein at mitochondria on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human liver carcinoma.
HMGCS2 antibody (GTX128013) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169