Wild-type (WT) and HN1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HN1 antibody (GTX106585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody (GTX106585) diluted at 1:3000.

An ELISA plate is coated with 50 μL of HN1 recombinant protein at concentration ranged from 0.004 μg/mL to 1 μg/mL. The coated protein is detected with HN1 antibody (GTX106585) at 0.25 μg/mL.

An ELISA plate is coated with 50 μL of HN1 recombinant protein at concentration ranged from 0.004 μg/mL to 1 μg/mL. The coated protein is detected with HN1 antibody (GTX106585) at 0.25 μg/mL.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody (GTX106585) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

An ELISA plate is coated with 50 μL of HN1 recombinant protein at concentration ranged from 0.004 μg/mL to 1 μg/mL. The coated protein is detected with HN1 antibody (GTX106585) at 0.25 μg/mL.