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HNF1 alpha antibody [GT4110]

Anti-HNF1 alpha antibody [GT4110] used in Western Blot (WB). GTX628240
Anti-HNF1 alpha antibody [GT4110] used in Western Blot (WB). GTX628240
Anti-HNF1 alpha antibody [GT4110] used in IHC (Paraffin sections) (IHC-P). GTX628240

Cat. No. GTX628240

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT4110

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HNF1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888023

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HNF1 homeobox A , HNF-1A , HNF1 , HNF4A , IDDM20 , LFB1 , MODY3 , TCF-1 , TCF1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HNF1 alpha antibody [GT4110] used in Western Blot (WB). GTX628240

GTX628240 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX628240 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HNF1 alpha antibody [GT4110] used in Western Blot (WB). GTX628240

GTX628240 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [GT4110] (GTX628240) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HNF1 alpha antibody [GT4110] used in IHC (Paraffin sections) (IHC-P). GTX628240

GTX628240 IHC-P Image

HNF1 alpha antibody [GT4110] detects HNF1 alpha protein at nucleus in human SW480 by immunohistochemical analysis.
Sample: Paraffin-embedded human SW480.
HNF1 alpha antibody [GT4110] (GTX628240) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lammi C et al. J Funct Foods 2016; 23 : 230-240 Lupin protein exerts cholesterol-lowering effects targeting PCSK9: From clinical evidences to elucidation of the in vitro molecular mechanism using HepG2 cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations