HNF1 alpha antibody [HL3229]

HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:100.
Antigen Retrieval: Tris-EDTA-Citrate acid buffer, pH 7.8, 15 min
Corresponding RNA expression data for the same tissues are based on NCBI.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HNF1 alpha antibody [HL3229] detects HNF1 alpha protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HNF1 alpha stained by HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [HL3229] (GTX640870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.