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HNF1 alpha antibody [N1N3]

Anti-HNF1 alpha antibody [N1N3] used in Immunoprecipitation (IP). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113850

Cat No. GTX113850

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

HepG2 , Mouse liver , Rat liver

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HNF1 homeobox A , HNF-1A , HNF1 , HNF4A , IDDM20 , LFB1 , MODY3 , TCF-1 , TCF1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HNF1 alpha antibody [N1N3] used in Immunoprecipitation (IP). GTX113850

GTX113850 IP Image

Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 μg of HNF1 alpha antibody [N1N3] (GTX113850).
Western blot analysis was performed using HNF1 alpha antibody [N1N3] (GTX113850).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha(GTX113850) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HNF1 alpha antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113850

GTX113850 ICC/IF Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169