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HNF1 alpha antibody [N1N3]

Orthogonal
Anti-HNF1 alpha antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in Immunoprecipitation (IP). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850
Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

Cat. No. GTX113850

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all HNF1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

rat liver

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HNF1 homeobox A , HNF-1A , HNF1 , HNF4A , IDDM20 , LFB1 , MODY3 , TCF-1 , TCF1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HNF1 alpha antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113850

GTX113850 ICC/IF Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha(GTX113850) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HNF1 alpha antibody [N1N3] used in Western Blot (WB). GTX113850

GTX113850 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HNF1 alpha antibody [N1N3] used in Immunoprecipitation (IP). GTX113850

GTX113850 IP Image

Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 μg of HNF1 alpha antibody [N1N3] (GTX113850).
Western blot analysis was performed using HNF1 alpha antibody [N1N3] (GTX113850).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HNF1 alpha antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113850

GTX113850 IHC-P Image

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HNF1 alpha stained by HNF1 alpha antibody [N1N3] (GTX113850) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Irene Ferrarese et al. J. Funct. Foods 2023; 108 : 105724 Bergamot (Citrus bergamia) peel extract as new hypocholesterolemic agent modulating PCSK9 expression
Application : WB
Reactivity : Human
Onuma S et al. Cells 2021; 10 (5) Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2021; 13 (3) Trans-Epithelial Transport, Metabolism, and Biological Activity Assessment of the Multi-Target Lupin Peptide LILPKHSDAD (P5) and Its Metabolite LPKHSDAD (P5-Met).
Application : WB
Reactivity : Human
Maruhashi R et al. Biochim Biophys Acta 2017; 1865 (3) : 470-479 Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models.
Application : WB
Reactivity : Human
Zanoni C et al. J Funct Foods 2017; 32 : 43108 Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways.
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REVIEW

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Application Reference
Irene Ferrarese et al. J. Funct. Foods 2023; 108:105724 Bergamot (Citrus bergamia) peel extract as new hypocholesterolemic agent modulating PCSK9 expression
Application : WB
Reactivity : Human
Onuma S et al. Cells 2021; 10 (5) Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2021; 13 (3) Trans-Epithelial Transport, Metabolism, and Biological Activity Assessment of the Multi-Target Lupin Peptide LILPKHSDAD (P5) and Its Metabolite LPKHSDAD (P5-Met).
Application : WB
Reactivity : Human
Maruhashi R et al. Biochim Biophys Acta 2017; 1865 (3):470-479 Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models.
Application : WB
Reactivity : Human
Zanoni C et al. J Funct Foods 2017; 32:43108 Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways.
Kowalczyk AE et al. Cancer Genomics Proteomics 2016; 13 (3):209-217 SATB1 is Down-regulated in Clear Cell Renal Cell Carcinoma and Correlates with miR-21-5p Overexpression and Poor Prognosis.
Application : WB
Reactivity : Human
Yasui K et al. Biomed Res 2011; 32 (5):313-20 Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum.
Application : WB
Reactivity : Human
Yasui K et al. J Med Food 2011; 14 (9):930-8 Effects of oolong tea on gene expression of gluconeogenic enzymes in the mouse liver and in rat hepatoma H4IIE cells.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169