*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , A549 nuclear extract
Mouse, Rat, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human HNF1 beta. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
HNF1 homeobox B , FJHN , HNF-1-beta , HNF-1B , HNF1beta , HNF2 , HPC11 , LF-B3 , LFB3 , MODY5 , TCF-2 , TCF2 , VHNF1
This gene encodes a member of the transcription factor superfamily. The encoded protein forms heterodimers with another member of this transcription factor family, HNF1A. Multiple alternatively spliced transcript variants have been described, however the biological validity all of these variants needs to be determined. [provided by RefSeq]
GTX129236 IHC-P Image
HNF1 beta antibody detects HNF1 beta protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
HNF1 beta antibody (GTX129236) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX129236 WB Image
A549 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 beta antibody (GTX129236) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.