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HNF4 alpha antibody, N-term

Anti-HNF4 alpha antibody, N-term used in Western Blot (WB). GTX89532
Anti-HNF4 alpha antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89532
Anti-HNF4 alpha antibody, N-term used in Western Blot (WB). GTX89532

Cat No. GTX89532

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
IHC-P 2μg/ml
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89532-PEP).

Product Note

This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1; NP_000448.3; NP_849181.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hepatocyte nuclear factor 4 alpha , FRTS4 , HNF4 , HNF4a7 , HNF4a8 , HNF4a9 , HNF4alpha , MODY , MODY1 , NR2A1 , NR2A21 , TCF , TCF14

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-HNF4 alpha antibody, N-term used in Western Blot (WB). GTX89532

GTX89532 WB Image

WB analysis of mouse small intestine lysate using GTX89532 HNF4 alpha antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-HNF4 alpha antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89532

GTX89532 IHC-P Image

IHC-P analysis of human small intestine using GTX89532 HNF4 alpha antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-HNF4 alpha antibody, N-term used in Western Blot (WB). GTX89532

GTX89532 WB Image

WB analysis of HepG2 lysate using GTX89532 HNF4 alpha antibody, N-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for HNF4 alpha antibody, N-term (GTX89532). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HNF4 alpha antibody, N-term (GTX89532). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 379