APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5μg/ml |
| 2μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89532-PEP).
Product Note
This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Predict Reactivity
Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1; NP_000448.3; NP_849181.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hepatocyte nuclear factor 4 alpha , FRTS4 , HNF4 , HNF4a7 , HNF4a8 , HNF4a9 , HNF4alpha , MODY , MODY1 , NR2A1 , NR2A21 , TCF , TCF14
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
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GTX89532 WB Image
WB analysis of HepG2 lysate using GTX89532 HNF4 alpha antibody, N-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89532 IHC-P Image
IHC-P analysis of human small intestine using GTX89532 HNF4 alpha antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
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GTX89532 WB Image
WB analysis of mouse small intestine lysate using GTX89532 HNF4 alpha antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
REVIEW
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