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HNF4 alpha antibody [SN72-03]

Anti-HNF4 alpha antibody [SN72-03] used in Western Blot (WB). GTX01036
Anti-HNF4 alpha antibody [SN72-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01036
Anti-HNF4 alpha antibody [SN72-03] used in IHC (Paraffin sections) (IHC-P). GTX01036
Anti-HNF4 alpha antibody [SN72-03] used in Flow cytometry (FACS). GTX01036
Anti-HNF4 alpha antibody [SN72-03] used in IHC (Paraffin sections) (IHC-P). GTX01036

Cat No. GTX01036

Host

Rabbit

Clonality

Monoclonal

Clone Name

SN72-03

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50-1:200
IHC-P 1:100-1:500
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FRTS4 , HNF4 , HNF4A , HNF4a7 , HNF4a8 , HNF4a9 , HNF4alpha , MODY , MODY1 , NR2A1 , NR2A21 , TCF , TCF14 , hepatocyte nuclear factor 4 alpha , HNF4 alpha , HNF4α

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-HNF4 alpha antibody [SN72-03] used in Western Blot (WB). GTX01036

GTX01036 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HNF4 alpha antibody [SN72-03] (GTX01036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HNF4 alpha antibody [SN72-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01036

GTX01036 ICC/IF Image

ICC/IF analysis of PFA-fixed SW480 cells using GTX01036 HNF4 alpha antibody [SN72-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-HNF4 alpha antibody [SN72-03] used in IHC (Paraffin sections) (IHC-P). GTX01036

GTX01036 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01036 HNF4 alpha antibody [SN72-03].

Anti-HNF4 alpha antibody [SN72-03] used in Flow cytometry (FACS). GTX01036

GTX01036 FACS Image

FACS analysis of HepG2 cells using GTX01036 HNF4 alpha antibody [SN72-03].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-HNF4 alpha antibody [SN72-03] used in IHC (Paraffin sections) (IHC-P). GTX01036

GTX01036 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01036 HNF4 alpha antibody [SN72-03].

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369