GeneTex
United States (US)

HNF4 alpha (phospho Ser313) antibody

Anti-HNF4 alpha (phospho Ser313) antibody used in IHC (Paraffin sections) (IHC-P). GTX32329
Anti-HNF4 alpha (phospho Ser313) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32329
Anti-HNF4 alpha (phospho Ser313) antibody used in Western Blot (WB). GTX32329

Cat No. GTX32329

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

53 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of HNF4 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hepatocyte Nuclear Factor 4 Alpha,Frts4,Hnf4,Hnf4A7,Hnf4A8,Hnf4A9,Hnf4Alpha,Mody,Mody1,Nr2A1,Nr2A21,Tcf,Tcf14,Hnf4A

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES
Anti-HNF4 alpha (phospho Ser313) antibody used in IHC (Paraffin sections) (IHC-P). GTX32329

GTX32329 IHC-P Image

IHC-P analysis of formalin fixed human colon cancer tissue section using GTX32329 HNF4 alpha (phospho Ser313) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-HNF4 alpha (phospho Ser313) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32329

GTX32329 ICC/IF Image

ICC/IF analysis of formalin-fixed SW480 cells using GTX32329 HNF4 alpha (phospho Ser313) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-HNF4 alpha (phospho Ser313) antibody used in Western Blot (WB). GTX32329

GTX32329 WB Image

WB analysis of SW480 (A), EGF-treated HepG2 (B) whole cell lysates using GTX32329 HNF4 alpha (phospho Ser313) antibody.

Package List Price ($)
$ 319