HNF4 alpha (phospho Ser313) antibody

Cat. No. GTX32329

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399)

See all HNF4 alpha products

Datasheet
Document

View product citations for antibody GTX32329 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of HNF4 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatocyte nuclear factor 4 alpha , FRTS4 , HNF4 , HNF4a7 , HNF4a8 , HNF4a9 , HNF4alpha , MODY , MODY1 , NR2A1 , NR2A21 , TCF , TCF14

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Database

Gene ID: 3172 HNF4A
UniProt: P41235 HNF4A

Research Area

DATA IMAGES

GTX32329 ICC/IF Image

ICC/IF analysis of formalin-fixed SW480 cells using GTX32329 HNF4 alpha (phospho Ser313) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32329 WB Image

WB analysis of SW480 (A), EGF-treated HepG2 (B) whole cell lysates using GTX32329 HNF4 alpha (phospho Ser313) antibody.

GTX32329 IHC-P Image

IHC-P analysis of formalin fixed human colon cancer tissue section using GTX32329 HNF4 alpha (phospho Ser313) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty