GeneTex
United States (US)

HNMT antibody

GTX54118_20180530_WB_18121410_424.jpg
GTX54118_20180530_ICCIF_18121410_910.jpg
GTX54118_20180530_IP_18121410_570.jpg

Cat No. GTX54118

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:200
ICC/IF 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histamine N-Methyltransferase,Hmt,Hnmt-S1,Hnmt-S2,Mrt51,Hnmt

Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX54118_20180530_WB_18121410_424.jpg

GTX54118 WB Image

WB analysis of various samples using GTX54118 HNMT antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX54118_20180530_ICCIF_18121410_910.jpg

GTX54118 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX54118 HNMT antibody.
Blue : DAPI

GTX54118_20180530_IP_18121410_570.jpg

GTX54118 IP Image

IP analysis of HT-29 cell lysate using GTX54118 HNMT antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Package List Price ($)
$ 319