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HNMT antibody

Anti-HNMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54118
Anti-HNMT antibody used in Immunoprecipitation (IP). GTX54118
Anti-HNMT antibody used in Western Blot (WB). GTX54118

Cat. No. GTX54118

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HNMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histamine N-methyltransferase , HMT , HNMT-S1 , HNMT-S2 , MRT51

Cellular Localization

Cytoplasm

Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HNMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54118

GTX54118 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54118 HNMT antibody.
Blue : DAPI

Anti-HNMT antibody used in Immunoprecipitation (IP). GTX54118

GTX54118 IP Image

IP analysis of HT-29 cell lysate using GTX54118 HNMT antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-HNMT antibody used in Western Blot (WB). GTX54118

GTX54118 WB Image

WB analysis of various sample lysates using GTX54118 HNMT antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399