*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88041-PEP).
This antibody is expected to recognize reported isoforms a and b (NP_005513.1; NP_705873.2).
Mouse, Rat, Bovine, Dog(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-QPATYQTSGN, from the internal region of the protein sequence according to NP_005513.1; NP_705873.2.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. The encoded protein may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Two transcript variants encoding two different isoforms have been found for this gene, with only one of the isoforms containing the homeodomain region. [provided by RefSeq, Jul 2008]
GTX88041 WB Image
WB analysis of HEK293 overexpressing human HOXA1 with C-terminal MYC tag using GTX88041 HOXA1 antibody, Internal.
Lane A : primary antibody
Lane B : Mock-transfected HEK293 probed with primary antibody
Lane C : Tag antibody
Dilution : 1µg/ml
Loading : 10µg protein in RIPA buffer