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HOXB13 antibody

Orthogonal
Anti-HOXB13 antibody used in Western Blot (WB). GTX129245
Anti-HOXB13 antibody used in Immunoprecipitation (IP). GTX129245
Anti-HOXB13 antibody used in Western Blot (WB). GTX129245
Anti-HOXB13 antibody used in Western Blot (WB). GTX129245

Cat. No. GTX129245

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HOXB13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

PC-3 nuclear extract

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HOXB13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885940

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

homeobox B13 , HPC9 , PSGD

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that belongs to the homeobox gene family. Genes of this family are highly conserved among vertebrates and essential for vertebrate embryonic development. This gene has been implicated to play a role in fetal skin development and cutaneous regeneration. In mice, a similar gene was shown to exhibit temporal and spatial colinearity in the main body axis of the embryo, but was not expressed in the secondary axes, which suggests functions in body patterning along the axis. This gene and other HOXB genes form a gene cluster at chromosome the 17q21-22 region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HOXB13 antibody used in Western Blot (WB). GTX129245

GTX129245 WB Image

Orthogonal

HOXB13 antibody detects HOXB13 protein by western blot analysis.
A. 30 μg PC-3 whole cell extract
B. 30 μg PC-3 nuclear extract
12 % SDS-PAGE
HOXB13 antibody (GTX129245) dilution: 1:1000

Anti-HOXB13 antibody used in Immunoprecipitation (IP). GTX129245

GTX129245 IP Image

Immunoprecipitation of HOXB13 protein from PC-3 nuclear extracts using 5 μg of HOXB13 antibody (GTX129245).
Western blot analysis was performed using HOXB13 antibody (GTX129245) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HOXB13 antibody used in Western Blot (WB). GTX129245

GTX129245 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31273254

Anti-HOXB13 antibody used in Western Blot (WB). GTX129245

GTX129245 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31273254

REFERENCE

Application Reference

Bakht MK et al. Nat Cancer 2023; 4 (5) : 699-715 Landscape of prostate-specific membrane antigen heterogeneity and regulation in AR-positive and AR-negative metastatic prostate cancer.
Paul Basil et al. Sci Rep 2022; 12 (1) : 5351 Cistrome and transcriptome analysis identifies unique androgen receptor (AR) and AR-V7 splice variant chromatin binding and transcriptional activities.
Nguyen NUN et al. Nature 2020; 582 (7811) : 271-276 A calcineurin-Hoxb13 axis regulates growth mode of mammalian cardiomyocytes.
Application : ChIP assay
Reactivity : Mouse
Yao J et al. Sci Rep 2019; 9 (1) : 9715 The Homeobox gene, HOXB13, Regulates a Mitotic Protein-Kinase Interaction Network in Metastatic Prostate Cancers.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169