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HOXB5 antibody

Anti-HOXB5 antibody used in Western Blot (WB). GTX129322
Anti-HOXB5 antibody used in Immunoprecipitation (IP). GTX129322

Cat No. GTX129322

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HepG2 WCE and NE

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HOXB5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

homeobox B5 , HHO.C10 , HOX2 , HOX2A , HU-1 , Hox2.1

Cellular Localization

Nucleus

Background

This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in lung and gut development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML) and the occurrence of bronchopulmonary sequestration (BPS) and congenital cystic adenomatoid malformation (CCAM) tissue. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HOXB5 antibody used in Western Blot (WB). GTX129322

GTX129322 WB Image

HOXB5 antibody detects HOXB5 protein by western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
12 % SDS-PAGE
HOXB5 antibody (GTX129322) dilution: 1:500

Anti-HOXB5 antibody used in Immunoprecipitation (IP). GTX129322

GTX129322 IP Image

Immunoprecipitation of HOXB5 protein from PC-3 nuclear extracts using 5 μg of HOXB5 antibody (GTX129322).
Western blot analysis was performed using HOXB5 antibody (GTX129322) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169