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HP1 alpha antibody [3G2-H10-A6]

Anti-HP1 alpha antibody [3G2-H10-A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49194
Anti-HP1 alpha antibody [3G2-H10-A6] used in Western Blot (WB). GTX49194

Cat. No. GTX49194

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G2-H10-A6

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all HP1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human CBX5 protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 5 , HEL25 , HP1 , HP1A

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HP1 alpha antibody [3G2-H10-A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49194

GTX49194 ICC/IF Image

ICC/IF analysis of HeLa cells using HP1 alpha antibody at a dilution of 1:300.

Anti-HP1 alpha antibody [3G2-H10-A6] used in Western Blot (WB). GTX49194

GTX49194 WB Image

WB analysis of indicated lysates using HP1 alpha antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399