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HP1 alpha antibody

Anti-HP1 alpha antibody used in Immunoprecipitation (IP). GTX53906
Anti-HP1 alpha antibody used in Western Blot (WB). GTX53906
Anti-HP1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53906

Cat No. GTX53906

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CBX5 (NP_001120794.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

chromobox 5 , HEL25 , HP1 , HP1A

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HP1 alpha antibody used in Immunoprecipitation (IP). GTX53906

GTX53906 IP Image

IP analysis of HeLa cells using GTX53906 HP1 alpha antibody.
IP reaction : 200μg extracts / 1μg antibody antibody
Dilution : 1:1000

Anti-HP1 alpha antibody used in Western Blot (WB). GTX53906

GTX53906 WB Image

WB analysis of various sample lysates using GTX53906 HP1 alpha antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-HP1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53906

GTX53906 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX53906 HP1 alpha antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf