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HP1 gamma antibody, Internal

Anti-HP1 gamma antibody, Internal used in Western Blot (WB). GTX89454
Anti-HP1 gamma antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89454

Cat. No. GTX89454

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all HP1 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 2-3μg/ml

Note :

IHC-P
Human Placenta shows strong nuclear staining in trophoblasts.
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89454-PEP).

Product Note

No cross-reactivity expected with HP1-alpha and HP1-beta.

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 3 , HECH , HP1-GAMMA , HP1Hs-gamma

Cellular Localization

Nucleus

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-HP1 gamma antibody, Internal used in Western Blot (WB). GTX89454

GTX89454 WB Image

WB analysis of A431 lysate using GTX89454 HP1 gamma antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-HP1 gamma antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89454

GTX89454 IHC-P Image

IHC-P analysis of human placenta using GTX89454 HP1 gamma antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399