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HP1 gamma antibody

KO/KD
Anti-HP1 gamma antibody used in Western Blot (WB). GTX117561
Anti-HP1 gamma antibody used in Immunoprecipitation (IP). GTX117561
Anti-HP1 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117561
Anti-HP1 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX117561
Anti-HP1 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX117561
Anti-HP1 gamma antibody used in Western Blot (WB). GTX117561
Anti-HP1 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX117561

Cat. No. GTX117561

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HP1 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HepG2 , HeLa

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HP1 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165271

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 3 , HECH , HP1-GAMMA , HP1Hs-gamma

Cellular Localization

Nucleus

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HP1 gamma antibody used in Western Blot (WB). GTX117561

GTX117561 WB Image

KO/KD

Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody (GTX117561) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HP1 gamma antibody used in Immunoprecipitation (IP). GTX117561

GTX117561 IP Image

HP1 gamma antibody immunoprecipitates HP1 gamma protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of HP1 gamma protein by 2 μg of HP1 gamma antibody (GTX117561)
15% SDS-PAGE
The immunoprecipitated HP1 gamma protein was detected by HP1 gamma antibody (GTX117561) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HP1 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117561

GTX117561 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HP1 gamma(GTX117561) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-HP1 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX117561

GTX117561 IHC-P Image

HP1 gamma antibody detects HP1 gamma protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HP1 gamma antibody (GTX117561) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HP1 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX117561

GTX117561 IHC-P Image

HP1 gamma antibody detects HP1 gamma protein at nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
HP1 gamma antibody (GTX117561) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HP1 gamma antibody used in Western Blot (WB). GTX117561

GTX117561 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX117561 diluted at 1:10000

Anti-HP1 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX117561

GTX117561 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using HP1 gamma(GTX117561) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Li Y et al. Nucleic Acids Res 2018; (Epub) Histone H1 acetylation at lysine 85 regulates chromatin condensation and genome stability upon DNA damage.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169