GeneTex
United States (US)

HPCAL1 antibody [N1C3]

GTX101816_WB_19011506_238.jpg
GTX101816_39721_WB_M_brain_19011506_701.jpg

Cat No. GTX101816

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HPCAL1 tansfected 293T cell , mouse brain

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HPCAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hippocalcin Like 1,Bdr1,Hlp2,Vilip-3,Hpcal1

Background

The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. There are two alternatively spliced transcript variants of this gene, with multiple polyadenylation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101816_WB_19011506_238.jpg

GTX101816 WB Image

Western Blot analysis of HPCAL1 expression in transfected 293T cell line by HPCAL1 polyclonal antibody.
A: Non-transfected lysate.
B: HPCAL1 transfected lysate.
12% SDS PAGE
GTX101816 diluted at 1:500

GTX101816_39721_WB_M_brain_19011506_701.jpg

GTX101816 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101816 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169