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HPCAL1 antibody [N1C3]

Overexpression
Anti-HPCAL1 antibody [N1C3] used in Western Blot (WB). GTX101816
Anti-HPCAL1 antibody [N1C3] used in Western Blot (WB). GTX101816
Anti-HPCAL1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101816

Cat. No. GTX101816

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all HPCAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brain , HPCAL1 transfected 293T

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HPCAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240957

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hippocalcin like 1 , BDR1 , HLP2 , VILIP-3

Background

The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. There are two alternatively spliced transcript variants of this gene, with multiple polyadenylation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HPCAL1 antibody [N1C3] used in Western Blot (WB). GTX101816

GTX101816 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101816 diluted at 1:1000

Anti-HPCAL1 antibody [N1C3] used in Western Blot (WB). GTX101816

GTX101816 WB Image

Overexpression

Western Blot analysis of HPCAL1 expression in transfected 293T cell line by HPCAL1 polyclonal antibody.
A: Non-transfected lysate.
B: HPCAL1 transfected lysate.
12% SDS PAGE
GTX101816 diluted at 1:500

Anti-HPCAL1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101816

GTX101816 IHC-P Image

HPCAL1 antibody [N1C3] detects HPCAL1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
HPCAL1 stained by HPCAL1 antibody [N1C3] (GTX101816) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169