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HPRT antibody [AT1D9]

Anti-HPRT antibody [AT1D9] used in Flow cytometry (FACS). GTX57727

Cat. No. GTX57727

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1D9

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all HPRT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1D9 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HPRT protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT , HPRT1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-HPRT antibody [AT1D9] used in Flow cytometry (FACS). GTX57727

GTX57727 FACS Image

FACS analysis of A549 cells using GTX57727 HPRT antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399