HPRT antibody [HL2015]

Wild-type (WT) and HPRT1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HPRT antibody [HL2015] detects HPRT protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole Japanese medaka extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.