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HPRT antibody [HL2015]

Recombinant antibody KO/KD Orthogonal
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916
Anti-HPRT antibody [HL2015] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637916
Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916

Cat. No. GTX637916

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2015

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all HPRT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human HPRT

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

KO/KD

Wild-type (WT) and HPRT1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916

GTX637916 IHC-P Image

HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Western Blot (WB). GTX637916

GTX637916 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody [HL2015] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637916

GTX637916 ICC/IF Image

HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HPRT antibody [HL2015] used in IHC (Paraffin sections) (IHC-P). GTX637916

GTX637916 IHC-P Image

HPRT antibody [HL2015] detects HPRT protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations