GeneTex
United States (US)

HPRT antibody

KO/KD
GTX101148_39911_20181109_WB_KO_watermark_19011519_554.jpg
GTX101148_39911_IFA_19011506_654.jpg
GTX101148_39911_IHC_19011506_368.jpg
GTX101148_39911_20160317_WB_R_brain_19011506_925.jpg
GTX101148_39911_20160121_WB_R_19011506_200.jpg
GTX101148_39911_20160317_WB_19011506_354.jpg

Cat No. GTX101148

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypoxanthine Phosphoribosyltransferase 1,Hgprt,Hprt,Hprt1

Cellular Localization

Cytoplasm

Background

HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101148_39911_20181109_WB_KO_watermark_19011519_554.jpg

GTX101148 WB Image

KO/KD

Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101148_39911_IFA_19011506_654.jpg

GTX101148 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using HPRT(GTX101148) antibody at 1:500 dilution.

GTX101148_39911_IHC_19011506_368.jpg

GTX101148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using HPRT(GTX101148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101148_39911_20160317_WB_R_brain_19011506_925.jpg

GTX101148 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:500.

GTX101148_39911_20160121_WB_R_19011506_200.jpg

GTX101148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:1000.

GTX101148_39911_20160317_WB_19011506_354.jpg

GTX101148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169