APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse liver , 293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037194
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT
Cellular Localization
Cytoplasm
Background
HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX113466 WB Image
Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113466 WB Image
HPRT antibody detects HPRT1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
12% SDS-PAGE
HPRT antibody (GTX113466) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113466 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113466 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX113466 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(GTX113466) antibody at 1:200 dilution.
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.HPRT stained by HPRT antibody (GTX113466) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113466 IHC-P Image
HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.HPRT stained by HPRT antibody (GTX113466) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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