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HPRT antibody

Anti-HPRT antibody used in Western Blot (WB). GTX113466
Anti-HPRT antibody used in Western Blot (WB). GTX113466
Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466
Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466
Anti-HPRT antibody used in Western Blot (WB). GTX113466
Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466
Anti-HPRT antibody used in Western Blot (WB). GTX113466
Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466
Anti-HPRT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113466
Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466

Cat No. GTX113466

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hypoxanthine phosphoribosyltransferase 1,HGPRT,HPRT

Cellular Localization

Cytoplasm

Background

HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-HPRT antibody used in Western Blot (WB). GTX113466

GTX113466 WB Image

Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody used in Western Blot (WB). GTX113466

GTX113466 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466

GTX113466 IHC-P Image

HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HPRT antibody (GTX113466) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466

GTX113466 IHC-P Image

HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HPRT antibody (GTX113466) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HPRT antibody used in Western Blot (WB). GTX113466

GTX113466 WB Image

HPRT antibody detects HPRT1 protein by western blot analysis.
A. 50 µg mouse liver lysate/extract
12% SDS-PAGE
HPRT antibody (GTX113466) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466

GTX113466 IHC-P Image

HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HPRT antibody (GTX113466) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HPRT antibody used in Western Blot (WB). GTX113466

GTX113466 WB Image

Sample (30 µg of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
12% SDS PAGE
GTX113466 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466

GTX113466 IHC-P Image

HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
HPRT antibody (GTX113466) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HPRT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113466

GTX113466 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(GTX113466) antibody at 1:200 dilution.

Anti-HPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX113466

GTX113466 IHC-P Image

HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
HPRT antibody (GTX113466) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169