GeneTex

  • Country / Location Selection

United States (US)

HPRT antibody

KO/KD
Anti-HPRT antibody used in Western Blot (WB). GTX33248
Anti-HPRT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33248
Anti-HPRT antibody used in Western Blot (WB). GTX33248

Cat. No. GTX33248

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HPRT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-HPRT antibody used in Western Blot (WB). GTX33248

GTX33248 WB Image

WB analysis of various sample lysates using GTX33248 HPRT antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HPRT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33248

GTX33248 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33248 HPRT antibody.
Blue : DAPI

Anti-HPRT antibody used in Western Blot (WB). GTX33248

GTX33248 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX33248 HPRT antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for HPRT antibody (GTX33248). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for HPRT antibody (GTX33248). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399