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HPRT antibody

Anti-HPRT antibody used in Western Blot (WB). GTX33248
Anti-HPRT antibody used in Western Blot (WB). GTX33248

Cat No. GTX33248

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hypoxanthine phosphoribosyltransferase 1 , HGPRT , HPRT

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-HPRT antibody used in Western Blot (WB). GTX33248

GTX33248 WB Image

WB analysis of various samples using GTX33248 HPRT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HPRT antibody used in Western Blot (WB). GTX33248

GTX33248 WB Image

WB analysis of wild type and HPRT1 knockout cell lysate using GTX33248 HPRT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329