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HPS3 antibody [C3], C-term

Anti-HPS3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107745
Anti-HPS3 antibody [C3], C-term used in Western Blot (WB). GTX107745
Anti-HPS3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107745

Cat. No. GTX107745

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HPS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HPS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240959

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HPS3 biogenesis of lysosomal organelles complex 2 subunit 1 , BLOC2S1 , SUTAL

Cellular Localization

Cytoplasm

Background

This gene encodes a protein containing a potential clathrin-binding motif, consensus dileucine signals, and tyrosine-based sorting signals for targeting to vesicles of lysosomal lineage. The encoded protein may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 3. Alternate splice variants exist, but their full length sequence has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HPS3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107745

GTX107745 ICC/IF Image

HPS3 antibody [C3], C-term detects HPS3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPS3 protein stained by HPS3 antibody [C3], C-term (GTX107745) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-HPS3 antibody [C3], C-term used in Western Blot (WB). GTX107745

GTX107745 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431 (GTX27909)
C:H1299
D:HeLa S3 (GTX14654)
E:Hep G2 (GTX27900)
F:MOLT4 (GTX27912)
G:Raji (GTX27908)
7.5% SDS PAGE
GTX107745 diluted at 1:1000

Anti-HPS3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107745

GTX107745 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HPS3(GTX107745) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169