GeneTex
United States (US)

HPS3 antibody [C3], C-term

GTX107745_IHC_19011507_981.jpg
GTX107745_40268_IFA_19011507_416.jpg
GTX107745_WB_19011507_913.jpg

Cat No. GTX107745

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HPS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hps3, Biogenesis Of Lysosomal Organelles Complex 2 Subunit 1,Bloc2S1,Sutal,Hps3

Cellular Localization

Cytoplasm

Background

This gene encodes a protein containing a potential clathrin-binding motif, consensus dileucine signals, and tyrosine-based sorting signals for targeting to vesicles of lysosomal lineage. The encoded protein may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 3. Alternate splice variants exist, but their full length sequence has not been determined. [provided by RefSeq]

Database

DATA IMAGES
GTX107745_IHC_19011507_981.jpg

GTX107745 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HPS3(GTX107745) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107745_40268_IFA_19011507_416.jpg

GTX107745 ICC/IF Image

HPS3 antibody [C3], C-term detects HPS3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPS3 protein stained by HPS3 antibody [C3], C-term (GTX107745) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX107745_WB_19011507_913.jpg

GTX107745 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431 (GTX27909)
C:H1299
D:HeLa S3 (GTX14654)
E:Hep G2 (GTX27900)
F:MOLT4 (GTX27912)
G:Raji (GTX27908)
7.5% SDS PAGE
GTX107745 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169