APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
| 2-4μg/ml |
|
Note :
IHC-P
Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89123-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence QQTSVRREDKCETD, from the internal region of the protein sequence according to NP_000852.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
histamine receptor H1 , H1-R , H1R , HH1R , hisH1
Cellular Localization
Cell membrane
Background
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2015]
Database
Research Area
DATA IMAGES
|
GTX89123 WB Image
WB analysis of human heart lysate using GTX89123 HRH1 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
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GTX89123 IHC-P Image
IHC-P analysis of human lung using GTX89123 HRH1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
REFERENCE
There are currently no references for HRH1 antibody, Internal (GTX89123). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HRH1 antibody, Internal (GTX89123). Be the first to share your experience with this product.
