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HRH1 antibody

Anti-HRH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102924
Anti-HRH1 antibody used in Western Blot (WB). GTX102924
Anti-HRH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102924

Cat No. GTX102924

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HRH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histamine receptor H1 , H1-R , H1R , HH1R , hisH1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HRH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102924

GTX102924 IHC-P Image

HRH1 antibody detects HRH1 protein at cytoplasm on human hepatocellular carcinomas by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinomas.
HRH1 antibody (GTX102924) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HRH1 antibody used in Western Blot (WB). GTX102924

GTX102924 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX102924 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HRH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102924

GTX102924 IHC-P Image

HRH1 antibody detects HRH1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
HRH1 antibody (GTX102924) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

HRH1 antibody Cat No. GTX102924

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 04-Jan-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 40 ug
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Lane Description : Lane 1: HEK293T cells, Lane 2-6: Different clones of HEK293T cell expressing human HRH1
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
Currently in production