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HRSP12 antibody

Anti-HRSP12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119107
Anti-HRSP12 antibody used in IHC (Paraffin sections) (IHC-P). GTX119107
Anti-HRSP12 antibody used in Western Blot (WB). GTX119107

Cat. No. GTX119107

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HRSP12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HRSP12 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

reactive intermediate imine deaminase A homolog , HRSP12 , P14.5 , PSP , UK114 , hp14.5

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES

Anti-HRSP12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119107

GTX119107 ICC/IF Image

HRSP12 antibody detects HRSP12 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: HRSP12 protein stained by HRSP12 antibody (GTX119107) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HRSP12 antibody used in IHC (Paraffin sections) (IHC-P). GTX119107

GTX119107 IHC-P Image

HRSP12 antibody detects HRSP12 protein at nucleus on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
HRSP12 antibody (GTX119107) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HRSP12 antibody used in Western Blot (WB). GTX119107

GTX119107 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
15% SDS PAGE
GTX119107 diluted at 1:2000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169